Escherichia coli

by Dr. K.A. Bettelheim

1) Introduction

2) What are E. coli?

3) E. coli in Natural Waters

4) E. coli in Starvation Conditions.

5) E. coli Survival under Stress.

6) E. coli in the Human Gut.

(a) Acquisition of E. coli by neonates.

(b) Adults also acquire E. coli.

(c) Microbiological Factors involved in acquiring E. coli.

7) E. coli in Domestic Animals.

(a) Pigs

(b) Cattle.

(c) Cats and Dogs.

(d) Rats and Mice.

(e) Domestic poultry.

(e) Domestic poultry.

(f) Birds.

(g) Fish

8) The Effect of Antibiotics on Intestinal E. coli.

(a) The development and transfer of resistance.

(b) The Behavior of resistant E.coli in the Human Intestine.

9. E. coli in the Food Chain.

(a) E. coli in the slaughter house.

(b) E. coli in food.

10. Biochemical Characteristics of Escherichia coli.

(a) Early Biochemical Studies.

(b) Carbon and Energy Sources.

(c) Nitrogen Sources.

(d) Metabolism of Glucose by E. coli.

(e) How E. coli can Respond to the Environment.

(f) Growth Rate.

(g)The Importance of Iron.

11. Diversity among Escherichia coli.

(a) The Clonal Nature of Escherichia coli.

(b) How Escherichia coli changes its Environment.

(c) Escherichia coli Serotypes.

(d) Escherichia coli Biotypes.

(e) Escherichia coli Phagetypes.

(f) Escherichia coli Colicintypes.

(g) Escherichia coli Pulsed-field gel Electrophoresis types.

(h) Conclusions

12. Genetics of Escherichia coli.

(a) Introduction

(b) The Escherichia coli Genome.

(c) Replication of the Escherichia coli Genome.

(d) RNA Synthesis in Escherichia coli.

(e) Synthesis of Ribosomal RNA.

(f) Regulation of Ribosomal RNA synthesis.

13. The Ribosome of Escherichia coli.

(a) The ribosomal proteins.

(b) The ribosomal RNA.

(c) Assembly of the Ribosomes.

(d) The Structure of Escherichia coli Ribosomes.

14. Protein Synthesis in Escherichia coli.

(a) The First Stage.

(b) The Second Stage.

(c) The Third Stage.

15. Human Intestinal Diseases caused by Escherichia coli.

General considerations.

16. Enteropathogenic Escherichia coli (EPEC).

(a) Introduction.

(b) Early Studies on Virulence of EPEC.

(c) Recent Studies on Virulence of EPEC.

(d) EPEC as Animal Pathogens.

(I) Pigs

(II) Cattle

(III) Sheep

(IV) Goats

(V) Horses

(VI) Poultry

(VII) Dogs

(VIII) Cats

(IX) Rabbits

17. Enterotoxigenic Escherichia coli (ETEC).

(a) Travellers' Diarrhoea.

(b) Military aspects of Travellers' Diarrhoea.

(c) Escherichia coli and Travellers' Diarrhoea.

(d) The Toxins of Enterotoxigenic Escherichia coli.

(e) The Heat-Labile Enterotoxin (LT) of Escherichia coli.

(f) The Heat-Stable Enterotoxin (ST) of Escherichia coli.

(g) Colonization Factors of ETEC.

(h) ETEC as Human Pathogens.

(i) ETEC as Animal Pathogens.

(I) Pigs

(II) Cattle

(III) Sheep

(IV) Goats

(V) Horses

(VI) Poultry

(VII) Dogs

(VIII) Cats

(IX) Rabbits

18. Enterohaemorrhagic Escherichia coli (EHEC).

(a) Vero or Shiga and Shiga-like Toxins.

(b) Mode of Action of the Verotoxins.

(c) Adherence Factors of the EHEC.

(d) The Haemolysin of EHEC.

(e) Ecology of EHEC.

(f) EHEC as Animal Pathogens.

(I) Pigs

(II) Cattle

(III) Sheep

(IV) Goats

(V) Horses

(VI) Poultry

(VII) Dogs

(VIII) Cats

(IX) Rabbits

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